A bacteriophage endolysin that eliminates intracellular streptococci
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چکیده
منابع مشابه
A bacteriophage endolysin that eliminates intracellular streptococci
PlyC, a bacteriophage-encoded endolysin, lyses Streptococcus pyogenes (Spy) on contact. Here, we demonstrate that PlyC is a potent agent for controlling intracellular Spy that often underlies refractory infections. We show that the PlyC holoenzyme, mediated by its PlyCB subunit, crosses epithelial cell membranes and clears intracellular Spy in a dose-dependent manner. Quantitative studies using...
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Campbell, Allan (University of Rochester, Rochester, N.Y.) and Alice del Campillo-Campbell. Mutant of lambda bacteriophage producing a thermolabile endolysin. J. Bacteriol. 85:1202-1207. 1963.-Endolysin from lambda bacteriophage and a temperature-sensitive mutant thereof was partially purified. The mutant enzyme was distinguishable from the wild type by its greater rate of inactivation by high ...
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The increasing spread of antibiotic-resistant pathogens has raised the interest in alternative antimicrobial treatments. In our study, the functionally active gram-negative bacterium bacteriophage CP933 endolysin was produced in Nicotiana benthamiana plants by a combination of transient expression and vacuole targeting strategies, and its antimicrobial activity was investigated. Expression of t...
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At least two genes have been shown to be required for the expression of the antiphagocytic M protein molecule in group A streptococci. Evidence for phage involvement in the expression of M protein is that: (a) M- cultures of bacteria can be converted to the M+ state (resistant to phagocytosis) upon lysogenization with appropriate bacteriophages; (b) without those bacteriophages the M- recipient...
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ژورنال
عنوان ژورنال: eLife
سال: 2016
ISSN: 2050-084X
DOI: 10.7554/elife.13152